Fig. 2.

Detection of myocardial cell apoptosis and in cardiac tissues from rats of different groups at T ₂. Apoptosis was determined by TUNEL assay according to manufacturer’s instructions. Cells with apoptotic morphologic features and with tan or brown nuclei were judged to be apoptotic cells. Groups C1 and C2: a small amount of apoptotic cells in myocardial tissue; groups I/R1 and I/R2: apoptotic cells in cardiac tissue increased significantly; groups LX1 and LX2: apoptotic cells were between group IR and group LX. Apoptosis index is represented by a histogram. ^* P < 0.05, comparisons of I/R1 and LX1 groups with C1 group; P < 0.05, comparisons of I/R2 and LX2 with C2 group; ^# P < 0.05, comparisons of LX1 group with I/R1 group, P < 0.05, comparisons of LX2 group with I/R2 group