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. 2013 Oct 16;29(5):667–678. doi: 10.1007/s00380-013-0418-y

Fig. 8.

Fig. 8

RT-qPCR analyses and Western blotting of myocardial GRP-78 and caspase-12 in rat cardiac tissues from the non-MIRI experiments. In the non-MIRI experiments, the rats were randomly divided into six groups (Sham1, Sham2, Sham3, Sham4, Sham5, and Sham6). The myocardial tissues were collected. The 150- and 90-min intervals before anesthesia mimicked the pretreatment and postprocessing times in these MIRI experiments.It was observed that there was no statistical difference among all groups. A Samples were collected and RT-qPCR was performed as described in Materials and methods. B Protein levels were detected by Western blotting. β-Actin was used as a loading control