Figure 3.
Stringent synchronization of cultures using Gdm-Cl. (a) Mature infected cells surviving synchronization with Gdm-Cl or sorbitol for indicated durations, as quantified using ring-stage parasitemia 24 h after treatment. A 5 min Gdm-Cl treatment is the most effective. (b) 72 h parasite growth after a 5 min treatment of synchronous cultures with Gdm-Cl, normalized to matched cultures sham-treated with culture medium. Bars represent mean ± S.E.M. of 9 replicates from 3 experiments.