Table 2.
Changes in the activities of superoxide dismutase, GSH-R, GST, paraoxonase, catalase, glutathione peroxidase and levels of both GSH and free radicals after treatment of male rats with a daily doses of dibuty-, ethylbutyl-, butylpropyl-, diethyl-, dimethyl- and diphenylnitrosamine for two weeks.
| Control | NDBA | NEBA | NBPA | NDEA | NDMA | NDPA | |
|---|---|---|---|---|---|---|---|
| GSH content μmole/gm liver |
1.8165 ± 0.39 | 2.268 ± 0.724 (+25%, P < 0.05)∗ |
2.184 ± 0.11 (+20%, P < 0.05)∗ |
2.227 ± 0.836 (+23%, P < 0.05)∗ |
2.21 ± 0.10 (+22%, P < 0.05)∗ |
2.516 ± 0.682 (+39%, P < 0.05)∗ |
2.091 ± 0.49 (+15%, P < 0.05)∗ |
|
| |||||||
| Free Radicals (TBARS) μmole/gm liver |
5.481 ± 0.35 | 10.856 ± 1.24 (+98%, P < 0.05)∗ |
8.35 ± 0.531 (+52%, P < 0.05)∗ |
7.952 ± 0.421 (+45%, P < 0.05)∗ |
7.552 ± 0.865 Ns |
12.68 ± 1.427 (+131%, P < 0.05)∗ |
7.618 ± 0.42 Ns |
|
| |||||||
| GSH-R activity nmolNADPH/mgprotein/min |
135 ± 5.12 | 269 ± 8.75 (+100%, P < 0.05)∗ |
194 ± 8.4 (+44%, P < 0.05)∗ |
181 ± 10.92 (+34%, P < 0.05)∗ |
208 ± 7.22 (+55%, P < 0.05)∗ |
208 ± 13.84 (+54%, P < 0.05)∗ |
139.39 ± 7.22 Ns |
|
| |||||||
| GST activity mmole/mgprotein/min |
0.335 ± 0.008 | 0.515 ± 0.023 (+54%, P < 0.05)∗ |
0.536 ± 0.016 (+60%, P < 0.05)∗ |
0.364 ± 0.199 Ns |
0.506 ± 0.027 (+51%, P < 0.05)∗ |
0.361 ± 0.013 Ns |
0.215 ± 0.006 (−36%, P < 0.05)∗ |
|
| |||||||
| Superoxide dismutase Units/g hemoglobin |
1.603 ± 0.231 | 0.837 ± 0.186 (−48%, P < 0.05)∗ |
1.460 ± 0.209 Ns |
0.769 ± 0.096 (−52%, P < 0.05)∗ |
1.985 ± 0.065 Ns |
1.20 ± 0.194 Ns |
0.772 ± 0.161 (−52%, P < 0.05)∗ |
|
| |||||||
| Paraoxonase activity μmole Paraoxonhydrolyzed/mg protein/min |
8.249 ± 0.600 | 9.527 ± 1.11 Ns |
15.173 ± 1.469 (+84%, P < 0.05)∗ |
11.577 ± 0.823 (+40%, P < 0.05)∗ |
14.90 ± 0.773 (+81%, P < 0.05)∗ |
18.234 ± 0.584 (+121%, P < 0.05)∗ |
12.22 ± 0.797 (+48%, P < 0.05)∗ |
|
| |||||||
| Catalase (U/gm tissue) | 3.05 ± 0.32 | 7.01 ± 0.56 (+131%, P < 0.05) |
8.14 ± 0.43 (+169%, P < 0.05) |
2.39 ± 0.18 NS |
2.02 ± 0.22 NS |
13.4 ± 0.61 (339%, P < 0.05) |
2.86 ± 0.36 NS |
|
| |||||||
| Glutathione peroxidase IU/gm Wet tissue |
69.29 ± 1.94 | 21.7 ± 1.11 (−69%, P < 0.05)∗ |
20.41 ± 0.99 (−71%, P < 0.05)∗ |
21.04 ± 0.6 (−70%, P < 0.05)∗ |
29.8 ± 0.75 (−57%, P < 0.05)∗ |
39.83 ± 2.26 (−43%, P < 0.05)∗ |
59 ± 2.93 (−15%, P < 0.05)∗ |
Values are expressed as Mean ± SEM.
NS: values are not significant statistically compared to the control group where P > 0.05.
∗Values are significantly differentcompared to control group where P < 0.05.