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. Author manuscript; available in PMC: 2015 Sep 1.
Published in final edited form as: J Biomol NMR. 2014 Aug 17;60(1):23–27. doi: 10.1007/s10858-014-9854-y

Figure 2.

Figure 2

Strips of the 3D H3NCG and H3CECD spectra for all Lys side-chain NH3+ groups of the Egr-1 – DNA complex at 10 °C. Negative contours are shown in green. The 1H and 15N spectral widths for were 18.0 ppm and 4.7 ppm, respectively, in both experiments. The 13C spectral widths were 20.7 ppm in H3NCG and 26.0 ppm in H3NCECD. In each experiment, 32 scans were accumulated per FID, and numbers of complex points for 1H, 13C, and 15N dimensions were 810, 32, and 32, respectively. The total experimental time was 60 hours each. These spectra were recorded at the 1H frequency of 750 MHz using a Bruker Avance III spectrometer equipped with a non-cryogenic TXI probe.