Table 2. Modulation of Clk/Sty Pre-mRNA Splicing by 16, 23, and 25 in Comparison to TG003.
| TG003 | 23 | 16 | 25 | |
|---|---|---|---|---|
| C5-fold (μM)a | 3.1 | 1.1 | 1.9 | 2.4 |
| EC50 (μM)b | 6.6 | 8.9 | 1.8 | 2.1 |
| max. inductionc | 17 | 21.4 | 9.5 | 8.1 |
a
Concentration required to induce a 5-fold increase of the mature Clk1 transcript levels as determined by qPCR; SD ≤ 10%.
b
Concentration required for half-maximal generation of the mature Clk1 mRNA splicing product; SD ≤ 10%.
c
Maximum increase of mature Clk1 transcripts achievable with the inhibitors (fold increase over DMSO-treated samples); SD< 20%. Shown are means of three independent experiments performed in triplicates.