Table 2. Inhibitory Activity of Peptide Hybrids against DENV and WNV Proteases and Thrombin^f.

^aPercent inhibition of DENV NS2B-NS3 protease serotype 2 (enzyme 100 nM, inhibitor 50 μM, substrate 50 μM, K_m = 105 μM).

^bIC₅₀ values against DENV NS2B-NS3 protease serotype 2 were determined for a substrate concentration of 50 μM.

^cPercent inhibition of WNV NS2B-NS3 protease (enzyme 150 nM, inhibitor 50 μM, substrate 50 μM, K_m = 212 μM).

^dPercent inhibition of thrombin (enzyme 10 nM, inhibitor 25 μM, substrate 50 μM, K_m = 16 μM).

^eCompound 9K_i at WNV NS2B-NS3 protease: 49 μM, IC₅₀ 58 μM. No inhibition of thrombin.

^fn.i. = no inhibition. All measurements were carried out in triplicate. Standard deviations were below 10%.