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. 2014 Sep 10;34(37):12490–12503. doi: 10.1523/JNEUROSCI.2238-14.2014

Figure 5.

Figure 5.

NOX2, but not NK1R, mediates the synergistic neurotoxicity of subpicomolar concentrations of SP in combination with LPS or MPP+, although NK1R is required for submicromolar SP-potentiated toxicity. A, B, Mesencephalic neuron-glia cultures prepared from WT (C57 BL/6J), NK1R−/−, and gp91phox−/− mice were cotreated with subpicomolar concentrations of SP and LPS (10 ng/ml) or (D, E) MPP+ (0.1 μm). Seven days later, the synergistic toxicity to dopaminergic neurons was quantified by (A, D) [3H]-DA uptake and (B, E) THir neuron counts (n = 5–10). C, F, Midbrain neuron-glia cultures prepared from WT and NK1R−/− mice were cotreated with submicromolar concentrations of SP (10−8 to 10−7 m) and (C) LPS (10 ng/ml) or (F) MPP+ (0.1 μm). Seven days later, the synergistic toxicity to dopaminergic neurons was quantified by [3H]-DA uptake. The results are expressed as the percentage of reduced [3H]-DA uptake capacity or loss of THir cells (mean ± SEM, control group was considered as 0, no damage). The data were analyzed using two-way ANOVA followed by Tukey's post hoc test. *p < 0.05. **p < 0.01.