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. 2014 Sep 10;34(37):12547–12559. doi: 10.1523/JNEUROSCI.0324-14.2014

Figure 1.

Figure 1.

Temporal profile of the hippocampal protein synthesis requirement during memory consolidation. Experimental schedule is shown above each figure. A–G, Acquisition (Acq.) and retention are expressed as mean latency ± SEM (in seconds) of rats given bilateral hippocampal injections (arrow) of vehicle or anisomycin at designated time points. A, Hippocampal injections of anisomycin 15 min before IA training had no effect on short-term memory tested at 1 h after training (n = 8/group, Test 1, vehicle vs anisomycin, p > 0.05, Student's t test). B, Hippocampal injections of anisomycin 15 min before IA training disrupted memory tested 2 and 7 d after training (n = 8–9/group, Test 1 and Test 2, vehicle vs anisomycin, p < 0.01, two-way ANOVA followed by Bonferroni post hoc tests; treatment effect F(1,28) = 22.37, p < 0.0001) as well as 1 d after a reminder footshock (Test 3, vehicle vs anisomycin, p < 0.01, Student's t test). Memory retention at Test 1, Test 2, or Test 3 was not significantly different from acquisition latency (p > 0.05, Student's t test). C, Hippocampal injections of anisomycin immediately after IA training disrupted memory tested 2 and 7 d after training (n = 8/group, Test 1 and Test 2, vehicle vs anisomycin, p < 0.05, two-way ANOVA followed by Bonferroni post hoc tests; treatment effect F(1,28) = 13.79, p = 0.0009) as well as 1 d after a reminder footshock (Test 3, vehicle vs anisomycin, p < 0.05, Student's t test). Memory retention at Test 1 was significantly different from acquisition latency (p < 0.05, Student's t test). D, Hippocampal injections of anisomycin 1 d after IA training had no effect on memory tested 2 d after the injection (n = 10/group, Test 1, vehicle vs anisomycin, p > 0.05, two-way ANOVA followed by Bonferroni post hoc tests), but significantly disrupted memory retested at 7 d after the injection (Test 2, p < 0.05, two-way ANOVA followed by Bonferroni post hoc tests; treatment effect F(1,36) = 5.277, p = 0.0275). Student's t test comparing Test 2 of the anisomycin group with its relative acquisition latency shows a statistically significant difference (Student's t test, p = 0.0210). E, Hippocampal injections of anisomycin 1 d after IA training significantly disrupted memory tested at 7 d after the injection (n = 8/group, Test 1, vehicle vs anisomycin, p < 0.05, Student's t test). Student's t test comparing Test 1 of the anisomycin group with its relative acquisition latency shows a statistically significant difference (Student's t test, p = 0.0412). F, Hippocampal injections of anisomycin 2 d after training had no effect on memory tested 2 d (Test 1) or 7 d (Test 2) after the injection (n = 12–14/group, Test 1 and Test 2, vehicle vs anisomycin, p > 0.05, two-way ANOVA followed by Bonferroni post hoc tests for both experiments). G, Hippocampal injections of anisomycin 7 d after training had no effect on memory tested 2 d (Test 1), 7 d (Test 2), or 21 d (Test 3) after the injection (n = 8–9/group, Test 1, Test2, and Test 3, vehicle vs anisomycin, p > 0.05, two-way ANOVA followed by Bonferroni post hoc tests); *p < 0.05, **p < 0.01.