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. Author manuscript; available in PMC: 2014 Sep 11.
Published in final edited form as: Nature. 2010 Oct 3;468(7322):406–411. doi: 10.1038/nature09428

Figure 5.

Figure 5

Remodeling of a G•T wobble base pair by AlkD. a, AlkD/G•T-DNA complex viewed down the helical axis. b, The structure of a G•T wobble base pair in DNA alone (PDB 113D) is superimposed onto the AlkD/G•T complex. Steric clashes between the protein and DNA are highlighted by yellow stars, and disrupted hydrogen bonds (dashed lines) are shown by a red X. c, Relative single-turnover rates (kst) of 7mG excision from a 25mer oligonucleotide duplex by wild-type and mutants of AlkD. Wild-type, D113N, and R148A data from ref. 19. Error bars represent the standard deviation from three independent measurements.