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1H-NMR spectra demonstrating production of Fos by HppE upon its reaction with H2O2. A, commercial Fos standard. B, products following slow addition of H2O2 (final concentration of 5.0 mM) to a solution of HppE (final concentration of 0.10 mM) FeII (0.080 mM), S-HPP (5.0 mM) and L-ascorbic acid (4.0 mM). The reaction was carried out at 21 °C in the absence of O2. C, the S-HPP substrate. Details of sample preparation in B and spectral acquisition are provided in supplementary materials.
