Table 1. Primers used for PCR amplification of four canine MC4R fragments.

Primer symbol	Primer sequence (5’-3’)	SNP	Amino acid change	Length
MC4R-1	F: CCTAGACTAAAGTTAAGGTGGGA	NO	NO	432 bp
	R: AGGGACTCAGTGGCGTTGC
MC4R-2	F: ACGCCACTGAGTCCCT	A420C	N101T	492 bp
	R: TGTCCGAGTAAATGATGAAC
MC4R-3	F: CATCATTTACTCGGACAG	C895T	NO	419 bp
	R: CACCCAGAGGATAGCA
MC4R-4	F: ACTCCATCATCGACCCT	NO	NO	232 bp
	R: TCTCAACTCCTGCCTTAT