Fig. 1.

Western blot analysis. A: Immunoblots of heart extracts prepared from wt, mdx, and DMD-null mice. Note: Both Dp427 and Dp71 were present in the hearts of wt mice, only Dp71 was present in the mdx mice, and neither was present in the DMD-null mice. The asterisk indicates a nonspecific signal. B: Immunoblots of subcellular fractions after separating heart homogenates from wt mice by iodixanol density-gradient centrifugation. Note: Dp427 was present in the cardiac sarcolemmal (#3, 4) and intracellular membrane fractions (#6. 7), while Dp71 was present in intracellular membrane fractions (#6. 7).