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. 2013 Aug 1;62(3):237–245. doi: 10.1538/expanim.62.237

Table 1. Primers used to detect MNV by RT-nested PCR.

Primer Sequence (5’ to 3’) Polarity1) Positions2)
1st PCR MNoVorf1&2-F5 CGCTTYGGAACRATGGATGCTG + 5001 − 5022
MNoVorf1&2-R2 AGCCRGTRTACATGGCTGAG 5340 − 5359
2nd PCR MNoVorf1&2-F6 CGCAGGAACGCTCAGCAGTC + 5029 − 5048
MNoVorf1&2-R3 CRAGRTARGGGTTRAGYCCYG 5312 − 5332

1)+, sense; −, anti-sense. 2)Nucleotide positions correspond to those of the MNV S7 complete genome (AB435514).