Table 1. Primers used to detect MNV by RT-nested PCR.
Primer | Sequence (5’ to 3’) | Polarity1) | Positions2) | |
---|---|---|---|---|
1st PCR | MNoVorf1&2-F5 | CGCTTYGGAACRATGGATGCTG | + | 5001 − 5022 |
MNoVorf1&2-R2 | AGCCRGTRTACATGGCTGAG | − | 5340 − 5359 | |
2nd PCR | MNoVorf1&2-F6 | CGCAGGAACGCTCAGCAGTC | + | 5029 − 5048 |
MNoVorf1&2-R3 | CRAGRTARGGGTTRAGYCCYG | − | 5312 − 5332 |
1)+, sense; −, anti-sense. 2)Nucleotide positions correspond to those of the MNV S7 complete genome (AB435514).