Table 3. Detection of MNV in feces of mice that drank WAHS1) post MNV infection.
| Drinking water | Weeks post infection |
|||||
|---|---|---|---|---|---|---|
| 1 | 2 | 3 | 4 | 5 | ||
| WAHS (n=3) | RT-nested PCR | 3/34) | 3/3 | 3/3 | 3/3 | 3/3 |
| MNV titer3) (log10 PFU/g) | 5.29 ± 0.53 | 3.79 ± 0.34 | 3.69 ± 1.10 | 3.89 ±1.01 | 3.74 ± 0.82 | |
| Control (n=3)2) | RT-nested PCR | 3/3 | 3/3 | 3/3 | 3/3 | 3/3 |
| MNV titer (log10 PFU/g) | 5.52 ± 0.54 | 3.51 ± 0.79 | 3.38 ± 0.57 | 3.77 ± 1.24 | 3.95 ± 0.85 | |
1)Drinking water was changed from 1 week post infection. 2)The drinking water of control mice was not changed to disinfectant. 3)MNV titer was determined by plaque assay. Each value represents the mean ± SD of 3 samples. For calculation of the mean ± SD, titers of samples that did not show any plaque were estimated as the detection limit, 3.0 log10. 4)Each value represents number of positive samples / number of samples examined.