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. 2013 Oct 31;62(4):319–328. doi: 10.1538/expanim.62.319

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©2013 Japanese Association for Laboratory Animal Science

This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License.

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Fig. 4. — TCR Vβ repertoire of NKT cells. NKT cells were isolated from the spleen, peripheral blood and epididymal, perirenal and subcutaneous adipose tissues. Evaluation of TCR Vβ repertoire was performed by flow cytometry. The antibodies used were anti-CD3ε (145-2C11), anti-NK1.1 (PK136) and anti-Vβ2 (B20.6), Vβ3 (KJ25), Vβ4 (KT4), Vβ5 (MR9-4), Vβ6 (44-22-1), Vβ7 (TR310), Vβ8.1, 8.2 (KJ-16), Vβ9 (MR10-2), Vβ10b (KT10b), and Vβ11 (RR3-15) (n=5).