RT-PCR analysis of canine CAT1 mRNA in various dog tissues (A).
Integrity of RNA was examined uing glyceraldehyde-3-phosphate dehydrogenase
(GAPDH). Lane 1, nictitating membrane gland; lane 2, lacrimal
grand; lane 3, LECs; lane 4, cerebellum; lane 5, trachea; lane 6, kidney; lane 7,
liver; lane 8, salivary gland; lane 9, lung; lane 10, testis; lane 11, heart; lane 12,
ileum; lane 13, colon; lane 14, bladder; lane 15, skeletal muscle; lane 16, placenta.
Western blot analysis of canine CAT1 expression in the LEC membrane (20
µg) using anti-human CAT1 antiserum (B).