Table 1. Sequences of oligonucleotides used in this study.
Primer | Sequence (5′-3′) | Expected product (bp) | |
Oligonucleotide for cloning of canine CAT1 | |||
s1 | Sense | CGGGCTCAGCITACCTCTACAGCT | |
a1 | Antisense | CAAAGAGGAAGGCCATCACAGCAGC | |
(Human CAT1: BC063303) | |||
GSP1 (for 5′ RACE) | Antisense | GTCCGCGAGAACTCCGCAATGGGTTTGC | |
GSP2 (for 3′ RACE) | Sense | AAGTATGCAGTGGCCGTCGGCTCCCTCT | |
Oligonucleotide for RT-PCR | |||
Transcript | |||
CAT1 (AB762780) | Sense | CGGGCTCAGCITACCTCTACAGCT | 392 |
Antisense | AGTGCCAATGGACATGAGGTCCACCA | ||
GAPDH (AB038240) | Sense | ATC ACC ATC TTC CAG GAG CGA GA | 192 |
Antisense | GTC TTC TGG GTG GCA GTG ATG G |
The accession number is indicated in parentheses.