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. 2014 Sep 11;5:469. doi: 10.3389/fmicb.2014.00469

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Copyright © 2014 Lapp, Vödisch, Kroll, Strassburger, Kniemeyer, Heinekamp and Brakhage.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Transcription of genes involved in RNI detoxification. (A) Northern blot analysis of fhpA and gnoA mRNA steady state levels in A. fumigatus cultivated for 16 h in AMM with different nitrogen sources. Gln, glutamine; rRNA was used as loading control. (B) PCR on cDNA of lungs of mice infected with A. fumigatus. Immunosuppressed mice were infected with A. fumigatus wild-type conidia and sacrificed at day 5 post infection. Genomic DNA (gDNA) of A. fumigatus was used as control template and transcription of the gene encoding A. fumigatus actin (act1) was used as positive control.