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. 2014 Sep 11;4:246. doi: 10.3389/fonc.2014.00246

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Copyright © 2014 Mohamed, El-Shinawi, Nouh, Bashtar, Elsayed, Schneider and Mohamed.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Incidence of detection of mixed HCMV different gB and gN genotypes in non-IBC and IBC carcinoma tissues is shown. (A) Agarose gel electrophoresis for nested multiplex PCR showed mixed presence of different HCMV gB genotypes. Lane C− represents negative control; lanes 1 and 2 represent gB-1 + gB-2 (420 bp + 613 bp) in non-IBC and IBC, respectively. Lanes 3 and 4 represent gB-1 + gB-3 (420 bp + 190 bp) non-IBC and IBC, respectively. Lanes 5 and 6 represent gB-1 + gB-4 (420 bp + 465 bp) in non-IBC and IBC, respectively. Lanes 7 and 8 represent gB-2 + gB-3 (613 bp + 190 bp) in non-IBC and IBC, respectively. Lanes 9 and 10 represent gB-2 + gB-4 (613 bp + 465 bp) in non-IBC and IBC, respectively. (B) Agarose gel electrophoresis for multiplex PCR showed mixed infection with different HCMV gN genotypes. Lane C− represents negative control, lanes 1 and 2 represent gN-1 + gN-2 (283 bp + 380 bp) in non-IBC and IBC, respectively, lanes 3 and 4 represent gN-1 + gN-3b (283 bp + 214 bp) in non-IBC and IBC, respectively, lanes 5 and 6 represent gN-1 + gN-4b/c (283 bp + 244 bp) in non-IBC and IBC, respectively, lanes 7 and 8 represent gN-2 + gN-4b/c (380 bp + 244 bp) in non-IBC and IBC, respectively, and lanes 9 and 10 represent gN-3b + gN-4b/c (214 bp + 244 bp) in non-IBC and IBC, respectively. (C) Bars represent the incidence of mixed infection with different HCMV gB genotypes in non-IBC and IBC carcinoma tissues as detected by multiplex PCR. (D) Bars represent the incidence of mixed infection with different HCMV gN genotypes. Non-IBC bar represents the percentage of mixed gN genotypes infections in non-IBC and IBC carcinoma tissues as detected by multiplex PCR. *Indicates a significant p value as determined by Fisher’s exact test.