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Forelimb malformation in 12-week-old mice. Alcian blue stains cartilage and alizarin red stains ossified bone (A1 and B1). Mutant forelimb show ectopic ossification (arrows) (B1). Soft X-ray (A2 and B2) Calcification was observed in and around the forelimb joints, and ectopic ossification was detected in the carpal bones (arrowhead) in the mutant mice (B1 and B2).
