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. 2014 Sep 11;5:312. doi: 10.3389/fgene.2014.00312

Figure 1.

Figure 1

Measuring gene expression in threespine stickleback kidneys. (A) Sampling locations in Bodega Bay and Davis, California, USA. (B) Hierarchical clustering of expression profiles for 2000 most variably expressed genes in samples, with a heat map chart showing 500 genes. (C) Expression profile of MAP3K15. The expression levels of MAP3K15 under the four acclimation conditions: FW00K, FW11K, FW33K, and SW33K, each with three replicates, are shown. The y-axis indicates the value of FPKM represented as the means ± s.e.m. The open square symbols indicate the mean FPKM across three replicates in same condition. The dash line links highlight the change in expression pattern of FPKM values across acclimation conditions. (D) RNA-seq and qRT-PCR results of relative expression differences (i.e., fold changes) between FW00K and FW33K for 10 select genes.