Skip to main content
NCBI home page
Infection and Immunity logoLink to Infection and Immunity
. 1971 Jan;3(1):10–15. doi: 10.1128/iai.3.1.10-15.1971

Occurrence of Protein A in Staphylococcal Strains: Quantitative Aspects and Correlation to Antigenic and Bacteriophage Types

Göran Kronvall 1,2, John H Dossett 1,2, Paul G Quie 1,2, Ralph C Williams Jr 1,2
PMCID: PMC416100  PMID: 16557923

Abstract

Protein A of Staphylococcus aureus can be detected on cell walls of intact bacteria by use of radioactively labeled myeloma globulin. Of 156 strains of S. aureus, 141 (90%) contained protein A. None of 47 S. epidermidis strains was positive for protein A. The production of protein A was influenced by incubation temperature but not by differences in incubation time or inoculum size. A medium containing a high concentration of NaCl suppressed the production of protein A by 90%. Formalin treatment of protein A-containing strains caused a decrease in the amount detected, but no further decrease was detected after storage at 4 C. No correlation was found between absence or presence of protein A and phage type or phage group. Sixteen S. aureus strains were studied extensively. There was no correlation between protein A and any of the 26 antigenic characteristics which have been previously described in these strains.

Full text

PDF
10

Images in this article

13Image
on p.13

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Forsgren A., Sjöquist J. "Protein A" from S. aureus. I. Pseudo-immune reaction with human gamma-globulin. J Immunol. 1966 Dec;97(6):822–827. [PubMed] [Google Scholar]
  2. Grov A., Oeding P., Myklestad B., Aasen J. Reactions of staphylococcal antigens with normal sera, gamma G-globulins, and gamma G-globulin fragments of various species origin. Acta Pathol Microbiol Scand B Microbiol Immunol. 1970;78(1):106–111. doi: 10.1111/j.1699-0463.1970.tb04275.x. [DOI] [PubMed] [Google Scholar]
  3. HAUKENES G. SEROLOGICAL TYPING OF STAPHYLOCOCCUS AUREUS. 1. FACTOR A SERUM. Acta Pathol Microbiol Scand. 1963;59:205–212. doi: 10.1111/j.1699-0463.1963.tb01790.x. [DOI] [PubMed] [Google Scholar]
  4. HAUKENES G. SEROLOGICAL TYPING OF STAPHYLOCOCCUS AUREUS. 2. FACTOR B SERUM. Acta Pathol Microbiol Scand. 1963;59:213–219. doi: 10.1111/j.1699-0463.1963.tb01791.x. [DOI] [PubMed] [Google Scholar]
  5. HAUKENES G. SEROLOGICAL TYPING OF STAPHYLOCOCCUS AUREUS. 3. FACTOR AC AND C SERA. Acta Pathol Microbiol Scand. 1963;59:220–228. doi: 10.1111/j.1699-0463.1963.tb01792.x. [DOI] [PubMed] [Google Scholar]
  6. HAUKENES G. SEROLOGICAL TYPING OF STAPHYLOCOCCUS AUREUS. 4. FACTOR H SERUM. Acta Pathol Microbiol Scand. 1964;60:285–294. doi: 10.1111/apm.1964.60.2.285. [DOI] [PubMed] [Google Scholar]
  7. HAUKENES G. SEROLOGICAL TYPING OF STAPHYLOCOCCUS AUREUS. 5. FACTOR I AND K SERA. RE-EXAMINATION OF FACTOR E, M, AND N SERA. Acta Pathol Microbiol Scand. 1964;61:283–290. doi: 10.1111/apm.1964.61.2.283. [DOI] [PubMed] [Google Scholar]
  8. HAUKENES G. SEROLOGICAL TYPING OF STAPHYLOCOCCUS AUREUS. 6. ANTIBODIES OF IMMUNE SERA TO STRAINS COWAN I, COWAN II, COWAN III, WOOD 46, 670, 830, 1015, 5687, AND 6376. Acta Pathol Microbiol Scand. 1964;61:415–426. doi: 10.1111/apm.1964.61.3.415. [DOI] [PubMed] [Google Scholar]
  9. Haukenes G. Serological typing of Staphylococcus aureus. 7. Technical aspects. Acta Pathol Microbiol Scand. 1967;70(4):590–600. doi: 10.1111/j.1699-0463.1967.tb01328.x. [DOI] [PubMed] [Google Scholar]
  10. James A. M., Brewer J. E. A protein component of the cell surface of Staphylococcus aureus. Biochem J. 1968 Jun;108(2):257–262. doi: 10.1042/bj1080257. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. KUNKEL H. G. Zone electrophoresis. Methods Biochem Anal. 1954;1:141–170. doi: 10.1002/9780470110171.ch6. [DOI] [PubMed] [Google Scholar]
  12. Kronvall G., Frommel D. Definition of staphylococcal protein A reactivity for human immunoglobulin G fragments. Immunochemistry. 1970 Jan;7(1):124–127. doi: 10.1016/0019-2791(70)90036-4. [DOI] [PubMed] [Google Scholar]
  13. Kronvall G., Quie P. G., Williams R. C., Jr Quantitation of staphylococcal protein A: Determination of equilibrium constant and number of protein A residues on bacteria. J Immunol. 1970 Feb;104(2):273–278. [PubMed] [Google Scholar]
  14. Kronvall G., Seal U. S., Finstad J., Williams R. C., Jr Phylogenetic insight into evolution of mammalian Fc fragment of gamma G globulin using staphylococcal protein A. J Immunol. 1970 Jan;104(1):140–147. [PubMed] [Google Scholar]
  15. Kronvall G., Williams R. C., Jr Differences in anti-protein A activity among IgG subgroups. J Immunol. 1969 Oct;103(4):828–833. [PubMed] [Google Scholar]
  16. Lind I., Mansa B. Further investigation of specific and non-specific adsorption of serum globulins to Staphylococcus aureus. Acta Pathol Microbiol Scand. 1968;73(4):637–645. doi: 10.1111/j.1699-0463.1968.tb03221.x. [DOI] [PubMed] [Google Scholar]
  17. Martin R. R., White A. Prevention of staphylococcal bacteriophage activity by antigen A precipitins in human sera. J Bacteriol. 1968 Jun;95(6):2177–2181. doi: 10.1128/jb.95.6.2177-2181.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. McConahey P. J., Dixon F. J. A method of trace iodination of proteins for immunologic studies. Int Arch Allergy Appl Immunol. 1966;29(2):185–189. doi: 10.1159/000229699. [DOI] [PubMed] [Google Scholar]
  19. Nickerson D. S., Kazmierowski J. A., Kronvall G., Williams R. C., Jr, Quie P. G. Immune response to chronic osteomyelitis in the rabbit. Proc Soc Exp Biol Med. 1970 Jul;134(3):821–824. doi: 10.3181/00379727-134-34891. [DOI] [PubMed] [Google Scholar]
  20. Nickerson D. S., White J. G., Kronvali G., Williams R. C., Jr, Quie P. G. Indirect visualization of Staphylococcus aureus protein A. J Exp Med. 1970 May 1;131(5):1039–1047. doi: 10.1084/jem.131.5.1039. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. OEDING P., HAUKENES G. IDENTIFICATION OF STAPHYLOCOCCUS AUREUS ANTIGENS AND ANTIBODIES BY MEANS OF THE GEL PRECIPITATION TECHNIQUE. Acta Pathol Microbiol Scand. 1963;57:438–450. doi: 10.1111/j.1699-0463.1963.tb05112.x. [DOI] [PubMed] [Google Scholar]
  22. OEDING P. Serological typing of Staphylococci. Acta Pathol Microbiol Scand Suppl. 1952 Jun;93:356–365. [PubMed] [Google Scholar]
  23. Smith H. B., Farkas-Himsley H. The relationship of pathogenic coagulase-negative staphylococci to Staphylococcus aureus. Can J Microbiol. 1969 Aug;15(8):879–890. doi: 10.1139/m69-157. [DOI] [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES