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. Author manuscript; available in PMC: 2014 Sep 11.
Published in final edited form as: Cancer Biomark. 2014 Jan 1;14(1):43–53. doi: 10.3233/CBM-130376

Figure 1. Effect of Alexa488 modification on biological activity and binding activity of pandemic H1N1 influenza virus.

Figure 1

A. Varying amounts of Alexa488 succinimidyl ester were reacted with purified virions (8,000 HAU) of A/Oklahoma/3052/2009 (pdmH1N1). Excess reagent was dialyzed away and residual HA and NA activities were measured using turkey red blood cells and MUN substrate respectively.

B. Binding of viruses (5,000 HAU/ml) to the Glycan Array v4.0 after reaction with varying amounts of Alexa488 as indicated. The y axis is fluorescent signal (RFU) of virus bound to the individual glycans on the array. The glycans that bind (ID numbers on the x axis) are sorted from high to low at 100 μg Alexa.