Figure 3.
A seasonal H1N1 virus shows broader binding specificity than pandemic H1N1 virus to the CFG Glycan Array. Results for two concentrations were normalized to 10,000 HAU/ml, averaged, and sorted from high to low binding of the seasonal strain A/Oklahoma/447/2008 to α2-6 linked sialic acid, then α2-3 sialylated and sulfated glycans. The pandemic H1N1 virus A/Oklahoma/3052/2009 passaged only in MDCK cells shows rather restricted binding specificity to a subset of α2-6 sialylated glycans, but after egg passage several additional glycans are seen to bind.