FIG. 3.
Metalloprotease activity is inhibited by Dox-treated melanoma cells grown on different substrates. (A) Zymography assay of human melanoma cell lines SK-Mel-19, SK-Mel-103, and SK-Mel-147 treated with 1.2 μM of Dox (IC50) or NT. (B) Gelatinolytic activity of MMP-2 and -9 in human melanoma cell line SK-Mel-103 (C) Inhibition of MMP-2 activity of by Biotrak® assay in human melanoma cell line SK-Mel-103, whose culture medium was incubated with a fluorescent substrate. Treatment conditions were melanoma cells seeded on plastic, in monolayer (M); on type I collagen (M+Col); on dermal equivalent (M+Eq); and the dermal equivalent control with no melanoma cells (Eq). ***p<0.001. MMP, matrix metalloproteinase.