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. Author manuscript; available in PMC: 2014 Sep 11.
Published in final edited form as: Stem Cells. 2009 Jul;27(7):1548–1558. doi: 10.1002/stem.81

Figure 2.

Figure 2

Transfection and characterization of dual-reporter gene-labeled murine breast cancer 4T1 cells. (A): Gene structure of lentivirus carrying dual-reporter (rLuc-mRFP) gene driven by a constitutive human ubiquitin promoter. (B): Lentiviral transfection efficiency was 73.58% as determined by fluorescence-activated cell sorting. (C): Fluorescence microscope image demonstrated that labeled 4T1 cells uniformly express RFP in the cytosol. (D): Transfected 4T1 cells constitutively express renilla luciferase. (E): Ex vivo analysis showed a linear relationship between the cell number and bioluminescence imaging signal. Abbreviations: LV LTR, lentivirus long term repeat; pUb, ubiquitin promoter; p/s/cm2/sr, photons per second per square centimeter per steridian; RLU, relative light unit; rLuc-mRFP, renilla luciferase-monomeric red fluorescence protein; SIN LTV, self-inactivating lentiviral long terminal repeat.