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. Author manuscript; available in PMC: 2014 Sep 11.
Published in final edited form as: Cancer Res. 2007 Aug 1;67(15):7175–7183. doi: 10.1158/0008-5472.CAN-06-4623

Figure 3.

Figure 3

Localization of BRET signal from subcutaneous and deep tissue structures of a nude mouse implanted with cells constitutively overexpressing GFP2-RLUC8. A, CCD camera image of a representative mouse implanted with 5 × 105 GFP2-Rluc cells on the left shoulder (L) and the same number of GFP2-Rluc8 cells on the right flank (R). The mice were injected with 25-µg Clz400 substrate via tail vein and imaged using a 2-min image acquisition time. B, CCD camera image of a representative mouse injected with 2 × 106 GFP2-Rluc8 cells by tail-vein injection. Thirty minutes later, the mouse was injected with 75-µg Clz400 and imaged immediately using a 3-min acquisition time. Unlike cells that stably express GFP2-RLUC, both donor and acceptor signals from GFP2-Rluc8 expression can be measured from the lungs. For both (A) and (B), images were first captured using the GFP filter followed by the DBC filter after a single injection of Clz400.