FIGURE 7.
VEGF ELISA results for supernatants of ASCs, b.END-3 and co-cultures. Cells were seeded onto 2D (a) and 3D (c) polystyrene or Matrigel (b) and cultured for 72-h under normoxic conditions. Significantly higher levels of VEGF were seen in co-cultures of ASCs, and b.END-3 when compared to controls. Triplicate measurements were performed and the data are from a representative experiment. Data are presented as mean ± SEM. VEGF levels were normalized to the number of cells at the time of seeding, expressed as pg/mL per 106 cells. *p < 0.05.