Fig. 2. Automated synthesis of the polyalanine octadecapeptide. Fmoc-Ala7HmsbAla6HmsbAla4Val-OH (A). Analytical HPLC trace of crude product HPLC conditions: RP-C18, 30–50% B (0.1% TFA, 90% CH3CN) in 30 min. 1 mL min–1. (B) MALDI-MS of product (calculated mass [M + Na]+ = 1965.8 m/z [M + K]+ = 1981.8 m/z). (C) Circular dichroism of polyalanine containing backbone protection forming random coil (dotted). Aggregated beta-sheets of polyalanine (solid) without backbone protection.
