Figure 3. Expanded gB-8p-specific memory cells in old HSV-1 infected mice uniformly exhibit central memory phenotype.
Splenocytes (shown) or lymphocytes isolated from blood (not shown) from old HSV-1 infected mice with age-related expansions of gB-8p-specific memory CD8+ T-cells were stained with CD8, tetramer and a panel of surface markers (CD44, CD62L, Ly6C, CD127, CD122, CD43, CD27). The staining of tetramer+ (top panel) and tetramer- (bottom panel) CD8+ T-cells from a representative mouse is shown. The same phenotype was observed in CD8+ T-cells isolated from blood (not shown).