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. Author manuscript; available in PMC: 2015 Feb 1.
Published in final edited form as: Stem Cells. 2014 Feb;32(2):487–499. doi: 10.1002/stem.1534

Figure 3. SDF1 regulates levels of CK1α and consequential phosphorylation and expression of β-catenin.

Figure 3

A) SDF1(50 nM) treatment rapidly decreased S45 phosphorylation of β-catenin with little effect on S33, S37, and T41 phosphorylation resulting in increased total levels of β-catenin. B) SDF1 (50 nM) decreased CK1α levels in cultured CSPCs. C) Functional consequences of increased β-catenin levels were confirmed by demonstrating increased β-catenin-dependent activation of the TCF/Lef promoter luciferase reporter, TOPFLASH, and its mutant reporter, FOPFLASH after 25 nM SDF1 treatment for 24 hours. Values are the mean ± SE, n=3. * p <0.05 vs Veh.