FIG 2 .

Construction of chimeric viruses expressing AKAP7 proteins. (A) Schematic diagram of the mutant ns2 and chimeric AKAP7 viruses. The AKAP7 cDNAs for Flag-tagged full-length and N-terminally truncated CD and its histidine mutant were inserted in place of ns4. The numbers above the boxes indicate the locations of histidine residues critical for the function of ns2 or AKAP7 protein (histidine-to-arginine mutations are shown in the boxes). The location of the predicted NLS is also indicated. aa, amino acids. (B) Verification of the expression of MHV ns2 proteins and AKAP7 proteins. Rnasel^−/− BMM were infected (1 PFU/cell) for about 10 h, and total cellular proteins were harvested and subjected to SDS-PAGE and Western blot analysis. Cellular GAPDH was monitored as a loading and transfer control.