FIG 3 .

Cellular localization of the AKAP7s in different cell types. 17Cl-1 cells (A) and B6 BMM (B) were infected with chimeric MHV viruses expressing full-length AKAP7, N-terminally truncated AKAP7 (AKAP7^ΔNTD), the CD of AKAP7 (AKAP7^CD), or its mutant (AKAP7^{CD H185R}) and stained for the expression of ns2 and AKAP7 with monoclonal antibodies against ns2 or against murine AKAP7 CD peptide A. DAPI was used to visualize nuclei. Magnification for panels A and B, ×60. (C) 293T cells expressed AKAP7 or AKAP^CD from lentivirus vectors (indicated). Western blot detection of AKAP7 and the AKAP7 CD by immunoblotting with anti-AKAP7 CD antibody (peptide B) and with antibody against β-actin as a loading and transfer control is shown. (D) 293T cell lines expressing AKAP7 or the AKAP7 CD (as in panel C) were stained with a polyclonal antiserum against murine AKAP7 CD peptide A. Nuclei were visualized with DAPI staining. Magnification, ×63.