FIG 8 .

Measurement of caspase-1 activation and YopM translocation in 129 or Iqgap1^−/− mouse macrophages infected with Y. pseudotuberculosis. BMDMs from 129 or Iqgap1^−/− mice were primed with 100 ng/ml LPS for 18 h and left uninfected or infected for 90 min at an MOI of 30 with the indicated strains of Y. pseudotuberculosis. Alternatively, 129 or Iqgap1^−/− BMDMs were treated with 100 ng/ml LPS for 4 h and then exposed to 2.5 mM ATP for 1 h to serve as a positive control for activation of caspase-1. (A) Lysates were collected and processed by Western blotting for cleaved caspase-1 or β-actin. (B) Supernatants were collected and analyzed by ELISA to quantify levels of secreted IL-1β. (C) Lysates were collected and processed by Western blotting for YopM or β-actin. The data in panel B are average values ± the standard errors of the means from three independent experiments. **, P < 0.01 (compared to 32777ΔyopM-infected 129 BMDMs by one-way ANOVA).