FIG 1 .

A fully functional proteasome-ubiquitin system in endothelial cells is crucial for efficient clearance of intracellular S. pneumoniae. (A) Relative survival of internalized S. pneumoniae cells during proteasomal inhibition with MG132 or TET. The control is set at 100%, and the experimental conditions are related to this percentage. Each circle represents the relative intracellular survival in an individual experiment, and horizontal lines indicate the averages of results from all experiments (n = 6 and 3, respectively). P values = 0.174 and <0.05, respectively. Relative intracellular survival is defined as intracellular survival divided by the amount of the initial invasion. (B) Relative survival of internalized S. pneumoniae during inhibition of the activating E1 enzyme with the inhibitor UBEI-41. The control is set as 100%, and the experimental conditions are related to this percentage. Each circle is the relative intracellular survival in an individual experiment, and horizontal lines indicate the averages of results from all experiments (n = 6). P value < 0.05. Relative intracellular survival is defined as intracellular survival divided by the amount of the initial invasion.