TABLE 3 .
Analysis of cosmid insert end sequences
| Clone | BLASTx result fora: |
|||
|---|---|---|---|---|
| Forward read |
Reverse read |
|||
| Description | E value (% identity [no. positive/total]) |
Description | E value (% identity [no. positive/total]) |
|
| C122 |
Porphyromonas gingivalis (4-amino-4-deoxy-l-arabinose transferase) |
4e–5 (29 [40/139]) |
Cellvibrio japonicus Ueda107 (β-xylosidase) |
8e–136 (82 [131/162]) |
| C424 |
Cellvibrio sp. strain BR (DNA-directed DNA polymerase) |
1e–28 (69 [66/80]) |
Cellvibrio sp. strain BR (Glucuronate isomerase) |
2e–103 (91 [157/163]) |
| C762 |
Chthoniobacter flavus (putative PAS/PAC sensor protein) |
1e–86 (78 [151/171]) |
Sorangium cellulosum (hypothetical protein) |
2e–28 (54 [83/125]) |
| C1024 |
Cellvibrio sp. strain BR (glucuronate isomerase) |
2e–17 (95 [34/40]) |
Cellvibrio sp. strain BR (gluconolaconase) |
2e–46 (80 [85/96]) |
| C1088 |
Saccharophagus degradans (SSS sodium solute transporter superfamily) |
6e–61 (68 [123/150]) |
Cellvibrio sp. strain BR (auxin efflux carrier) |
5e–44 (75 [101/114]) |
| C2194 |
Dyadobacter fermentans (ROK family protein) |
1e–91 (95% [140/142]) | Failed sequencing reaction |
|
| C2380 |
Alicyclobacillus acidocaldarius (Glyoxalase/bleomycin resistance protein/dioxygenase) |
2e–15 (52 [51/69]) |
Cellvibrio sp. strain BR (glucosamine fructose-6-phosphate aminotransferase, isomerizing) |
3e–105 (96 [162/163]) |
| C2044 |
Cellvibrio sp. strain BR (DNA polymerase III subunit delta) |
1e–71 (96 [116/118]) |
Dyadobacter fermentans (hypothetical protein) |
9e–129 (97 [181/184]) |
a
Cosmids were end sequenced with M13 forward and reverse primers flanking the site of metagenomic DNA insertion. For each clone, two end sequences were obtained and are referred to as “reverse” and “forward” reads. Top matches for BLASTx analyses are shown. Positive results are the number of amino acids from the query that match the amino acids from the subject sequence. The total number of amino acids from the subject is shown.