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. 2014 Sep 11;10(9):e1004612. doi: 10.1371/journal.pgen.1004612

Figure 1. The rrp6l1-3 and rrp6l2-3 mutants affect flowering time and gene expression.

Figure 1

(A) The late-flowering phenotype of rrp6l1 and rrp6l2 mutants grown under long day conditions. Flowering time was measured as rosette leaf number at bolting. To control for effects of ecotype, the phenotype of mutants was compared to wild-type plants of Col-0 and Ws ecotypes. Error bars represent standard deviation (SD). (B) The late-flowering phenotype of rrp6l1-3 rrp6l2-3 mutants grown under short day conditions. 66-day-old plants are shown. (C) Effect of rrp6l1-3 and rrp6l2-3 mutations on the expression of the FLC, SOC1 and FT. RT-PCR showed that the rrp6l1-3 rrp6l2-3 double mutant (rrp6l1/2), has increased expression of FLC and decreased expression of SOC1 and FT, which act downstream of FLC. (-RT) is the no reverse transcriptase control. (D) The expression of FCA, FPA, FLD, FVE, FLM, and LDL2, genes involved in regulation of flowering time in the autonomous flowering pathway, is not affected in rrp6l1/2 mutants. (E) FLC expression is not affected in AtCSL4-2 T-DNA mutant and RRP41 iRNAi line. RRP41 corresponds to the iRNAi line grown without estradiol, and rrp41-i corresponds to line grown on estradiol-containing medium, to induce the RNAi-mediated knockdown of RRP41. TUBULIN 2 and ACTIN 7 were used as loading controls.