Table 1. Cell mediated activities in the DNA/Ad and AdCA trials at post-Ad time point (4–5 days before challenge).

	ELISpot sfc/m		ICS/Flow cytometry
			CD8+ %		CD8+ CM %		CD8+ EM %
	CSP	AMA1	CSP	AMA1	CSP	AMA1	CSP	AMA1
A. DNA/Ad
Protected volunteers
v06	80	312	<0.03	0.08	<0.03	0.04	<0.03	<0.03
v10	55	810	<0.03	0.22	<0.03	0.09	<0.03	0.07
v11	408	1046	0.21	0.98	0.08	0.21	0.05	0.46
v18	398	1270	0.10	0.52	0.06	0.17	<0.03	0.27
No. positive1/total number	2/4	4/4	2/4	3/4	2/4	3/4	1/4	3/4
Non-protected volunteers
Geometric mean	68	262	0.04	0.11	<0.03	0.05	<0.03	<0.03
Range	13–217	88–787	<0.03–0.293	0.05–0.19	<0.03–0.133	<0.03–0.16	<0.03–0.03	<0.03–0.07
No. positive1/total number	2/11	8/11	2/11	8/11	2/11	5/11	0/11	0/11
No. with higher activity than highest protected2	0/11	0/11	13/11	0/11	13/11	0/11	0/11	0/11
B. AdCA Non-protected
Geometric mean	273	1303	0.12	0.49	0.05	0.31	<0.03	0.08
Range	38–2550	435–4594	0.05–0.57	0.13–2.44	0–0.38	0.08–1.54	<0.03–0.09	<0.03–0.33
No. positive1/total number	14/18	18/18	13/16	16/16	8/16	15/16	3/16	10/16
No. with higher activity than highest protected2	5/18	11/18	5/16	2/16	5/16	11/16	3/16	0/164
C. Comparisons of geometric mean activities of non-protected volunteers
DNA/Ad Non-protected: ADCA Non-protected p value5	0.0018	<0.0001	0.0069	0.0002	0.0025	0.0001	0.0500	0.0014

¹A volunteer was considered positive for an assay if activity to one or more CSP or AMA1 peptide pools was positive (see Methods). For individual protected volunteers, positive activities are in bold.

²The number of volunteers with values higher than the highest value among the four protected DNA/Ad volunteers.

³Highest values are for v15.

⁴Two protected volunteers (v10 and v18) were lower than the highest AdCA activity.

⁵p value: non-protected volunteers were included in the comparison. Statistical comparisons are included as an exploratory analysis, since the different trials were not designed or powered for a formal statistical comparison. No.  =  Number.