Table 2. ELISpot responses to Ap8 and Ap10 peptide pools and individual 15mer peptides within each pool.
Pool | 15mer | Sequence | v102 | v111 | v182 | V1563 | V1943 |
Prot. | Prot. | Prot. | NP | Delay | |||
Ap8 | 193 | 321 | 141 | 47 | |||
A92 | EGFKNKNASMIKSAF | 1 | 0 | 0 | 4 | ||
A93 | NKNASMIKSAFLPTG | 0 | 2 | 0 | 4 | ||
A94 | SMIKSAFLPTGAFKA | 9 | 10 | 11 | 6 | ||
A95 | SAFLPTGAFK ADRYK | 9 | 2 | 61 | 4 | ||
A96 | PTGAFKADRYKSHGK | 1 | 2 | 0 | 3 | ||
A97 | FKADRYKSHGKGYNW | 164 | 270 | 141 | 48 | ||
A98 | RYKSHGKGYNW GNYN | 96 | 85 | 14 | 7 | ||
A99 | HGKGYNWGNYNTETQ | 0 | 5 | 5 | 6 | ||
A100 | YNWGNYNTETQKCEI | 1 | 5 | 0 | 6 | ||
A101 | NYNTETQKCEIFNVK | 0 | 0 | 1 | 0 | ||
A102 | ETQKCEIFNVKPTCL | 0 | 0 | 0 | 6 | ||
A103 | CEIFNVKPTCLINNS | 1 | 0 | 1 | 1 | ||
A104 | NVKPTCLINNSSYIA | 3 | 0 | 0 | 0 | ||
Ap10 | 159 | ||||||
A118 | EGNKKIIAPRIFISD | 2 | |||||
A119 | KIIAPRIFISDDKDS | 0 | |||||
A120 | PRIFISDDKDSLKCP | 1 | |||||
A121 | ISDDKDSLKCPCDPE | 3 | |||||
A122 | KDSLKCPCDPEMVSN | 2 | |||||
A123 | KCPCDPEMVSNSTCR | 1 | |||||
A124 | DPEMVSNSTCRFFVC | 0 | |||||
A125 | VSNSTCRFFVCK CVE | 140 | |||||
A126 | TCRFFVCKCVERRAE | 9 | |||||
A127 | FVCKCVERRAEVTSN | 4 | |||||
A128 | CVERRAEVTSNNEVV | 10 | |||||
A129 | RAEVTSNNEVVVKEE | 0 | |||||
A130 | TSNNEVVVKEEYKDE | 0 |
DNA/Ad cells collected 28 days after malaria challenge;
DNA/Ad cells collected 84 days after malaria challenge.
AdCA cells collected 2 months after the malaria challenge. Prot. = Protected. NP = Not Protected. All 15mer peptides within Ap8 and Ap10 were tested with DNA/Ad, and AdCA-immunized volunteers in ELISpot assays. Positive activities (bold): activity was greater than twice that of control, and the difference in absolute count was >10 sfc/m. Predicted epitopes within positive 15mers are shown in bold and underlined.