Figure 3. Refolding of sdAb.

Circular dichroism was used to monitor the secondary structure of purified sdAbs over a temperature range of 25–95°C. Representative plots are shown of clones H7 and H2 typifying the distinctly different refolding properties. Temperature is expressed in °C and the change in ellipticity is in milidegrees. CD experiments were performed in duplicate for most of the sdAbs; the observed Tm values agreed within a degree, and the refolding trends were the same among the replicate measurements.