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. 2014 Sep 11;9(9):e106263. doi: 10.1371/journal.pone.0106263

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This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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Fluorescence based melting assay showing melting of L1-G2 in blue and the mutant with the added disulfide, L1-G2+, in black. Melting temperature is determined from a plot of the first derivative of the melt curve. The measurements were performed in triplicate on two different days and showed identical results; for clarity only one replicate is shown.