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. 2014 Sep 11;9(9):e106263. doi: 10.1371/journal.pone.0106263

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This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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Direct binding assays were used to demonstrate target specificity for L1 binding antigens. Antigen was immobilized to magnetic beads and biotinylated (bt)- sdAbs (noted at the top of each graph) served as the tracers. Independent direct binding assays were performed different days; a representative data set is shown. The MAGPIX assays typically measures binding from at least 50 beads and the error of the mean fluorescence intensity (MFI) is ≤5%.