Table 3. Melting temperatures of L1 binding sdAbs and mAbs.

sdAb/mAb	Melting temperature (°C) (Fluorescence melt assay*)	Melting temperature (°C) (Circular dichroism**)
L1-C5	57	58
L1-E2	56	56
L1-E6	60	60
L1-G2	61	64
L1-G2 +	80	81
L1-H2	62	66
L1-H7	60	62
mAb 417	71	ND
mAb 418	71	ND
mAb 419	70	ND

ND  =  not determined.

* Fluorescent melt assays performed in triplicate on at least 2 days. The triplicate measurements were typically identical within less than 0.2°C, and always within 0.6°C, while samples measured from different protein lots or on different days agreed within a degree. ** Most of the CD assays were performed on at least two different days. Values determined by CD agreed within a degree.