Table 5. Determination of recombinant frequencies in PCR with 35 distinct templates by next generation sequencing.
Number of cycles | Copy of templates | Total raw reads | Merged reads | Filtered sequences | Number of sequences aligned to reference | Recombinant sequences | |
Number | Percentage | ||||||
30 | 3.5×105 | 291,206 | 288,721 | 200,665 | 188,891 | 24,833 | 13% |
3.5×106 | 406,988 | 403,870 | 277,553 | 262,456 | 40,078 | 15% | |
35 | 3.5×104 | 205,152 | 203,512 | 117,362 | 110,412 | 13,812 | 13% |
3.5×105 | 383,225 | 380,152 | 245,514 | 232,128 | 39,066 | 17% | |
3.5×106 | 349,839 | 347,025 | 226,771 | 214,915 | 75,407 | 35% | |
40 | 3.5×104 | 257,073 | 254,828 | 166,013 | 154,172 | 24,496 | 16% |
3.5×105 | 353,325 | 350,222 | 228,543 | 214,317 | 52,324 | 24% | |
3.5×106 | 300,406 | 298,262 | 204,089 | 192,880 | 85,584 | 44% | |
45 | 3.5×104 | 310,392 | 307,886 | 203,912 | 191,644 | 44,856 | 23% |
3.5×105 | 381,909 | 378,724 | 245,945 | 231,893 | 107,881 | 47% | |
3.5×106 | 343,394 | 340,447 | 194,522 | 184,538 | 112,661 | 61% |