Figure 8. The effect of simvastatin on eNOS and NO production.

A. Total eNOS expression normalised to GAPDH in control and simvastatin-treated cells. B. Ser¹¹⁷⁷-phosphorylated eNOS normalised to GAPDH in control and simvastatin-treated cells. Data are from cells from n = 5 hearts. C. Medium was collected from myocytes cultured for 48 h in the presence or absence of simvastatin. NO metabolites (nitrate/nitrite) were measured by a fluorimetric assay. Data are [nitrite + nitrate] normalised to values in paired control group (cells from n = 6 hearts). * P<0.05, Student's t-test.