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. 2014 Jul 23;15(13):1877–1881. doi: 10.1002/cbic.201402037

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© 2014 The Authors. Published by Wiley-VCH Verlag GmbH & Co. KGaA. This is an open access article under the terms of the Creative Commons Attribution Non-Commercial NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

© 2014 The Authors. Published by Wiley-VCH Verlag GmbH & Co. KGaA. This is an open access article under the terms of the Creative Commons Attribution Non-Commercial NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Progress of the LN12 DIS selection by flow cytometry. Bead-bound LN12 library annealed to A) the FITC oligonucleotide to determine the positive gates (“ON′′), or B) both the FITC and DABCYL oligonucleotides to sort quenched beads (”OFF“). C) DIS (200 μm) was added to quenched, sorted beads (gray) and re-sorted for positive events (”ON′′; orange). The negative sort was omitted in the first round. R0 data was derived from the initial LN12 library, R1 from the first enriched library, etc. Variations in the position of both the “ON” and “OFF” gates were due to day-to-day differences encountered on the flow cytometer.