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. 2014 Jul 23;15(13):1877–1881. doi: 10.1002/cbic.201402037

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© 2014 The Authors. Published by Wiley-VCH Verlag GmbH & Co. KGaA. This is an open access article under the terms of the Creative Commons Attribution Non-Commercial NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

© 2014 The Authors. Published by Wiley-VCH Verlag GmbH & Co. KGaA. This is an open access article under the terms of the Creative Commons Attribution Non-Commercial NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Scheme 2 — FACS-based selection. A) Microbeads bearing multiple copies (∼10⁵) of a forward primer and a single dsDNA member of the library are amplified by emulsion PCR (ePCR), generating B) beads bearing ∼40 000 copies of a single sequence. The emulsion is broken, and the beads are recovered and C) rendered single-stranded by treatment with NaOH. Single-stranded DNA is hybridized to fluorophore (F) and quencher (Q) oligonucleotides, generating libraries of particles in the “OFF” state. D) Following incubation with target, E) particles which turn “ON” in the presence of ligand can be recovered by F) FACS.