Table 1.
Examples of different MRU frequencies measured in suspensions of unfractionated fetal and adult mammary tissue and from different strains of mice and the effect of including Matrigel in the inoculum
| Stage | Mouse strain | Frequency of MRU (95 % CI) no Matrigel |
Frequency of MRU (95 % CI) +Matrigel |
Composition of inoculum solution |
References |
|---|---|---|---|---|---|
| Adult | FVB | 1/1,400 (1/600–1/3,000) | HF | [8] | |
| Adult | FVB | 1/106 (1/70–1/160) | PBS: Matrigel (1:1) | [85] | |
| Adult | BALB/c | 1/7,800 (1/1,900–1/31,000) | 5 μg/ml Matrigel | [37] | |
| Adult | BL/6J | 1/2,000 (1/900–1/4,500) | HF | [8] | |
| Adult | BL/6J × CD-1 | 1/30,000 (1/11,000–1/80,000) | 1/300 (1/150–1/500) | HF or HF: Matrigel (1:1) | [14] |
| Fetala | BL/6J × CD-1 | 1/800 (1/400–1/1,600) | 1/60 (1/40–1/80) | HF or HF: Matrigel (1:1) | [14] |
HF: 2 % FBS in Hanks’ Balanced salt solution
a
Similar frequencies obtained by Makarem et al. unpublished