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. Author manuscript; available in PMC: 2014 Sep 11.
Published in final edited form as: J Mammary Gland Biol Neoplasia. 2013 Apr 27;18(2):209–219. doi: 10.1007/s10911-013-9284-6

Table 1.

Examples of different MRU frequencies measured in suspensions of unfractionated fetal and adult mammary tissue and from different strains of mice and the effect of including Matrigel in the inoculum

Stage Mouse strain Frequency of MRU
(95 % CI) no Matrigel
Frequency of MRU
(95 % CI) +Matrigel
Composition of
inoculum solution
References
Adult FVB 1/1,400 (1/600–1/3,000) HF [8]
Adult FVB 1/106 (1/70–1/160) PBS: Matrigel (1:1) [85]
Adult BALB/c 1/7,800 (1/1,900–1/31,000) 5 μg/ml Matrigel [37]
Adult BL/6J 1/2,000 (1/900–1/4,500) HF [8]
Adult BL/6J × CD-1 1/30,000 (1/11,000–1/80,000) 1/300 (1/150–1/500) HF or HF: Matrigel (1:1) [14]
Fetala BL/6J × CD-1 1/800 (1/400–1/1,600) 1/60 (1/40–1/80) HF or HF: Matrigel (1:1) [14]

HF: 2 % FBS in Hanks’ Balanced salt solution

a

Similar frequencies obtained by Makarem et al. unpublished