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. 2014 Sep 11;9(9):e106901. doi: 10.1371/journal.pone.0106901

Figure 3. Cord phenotypes driven by angiogenic growth factors.

Figure 3

(A) ADSC and ECFC co-cultures were stimulated with angiogenic growth factors; VEGF, HGF, bFGF, or EGF and stained for cords (CD31; green), smooth muscle actin (SMA; red), and nuclei (Hoechst 33342; blue). (B) To assess whether the cord induction was dependent on VEGF, cords were treated with the different angiogenic factors plus an anti-VEGF antibody (Bevacizumab; Bev). (C) ArrayScan quantification of total tube area, branching index, length to width ratio and SMA index are shown as an indication of phenotypes associated with the VEGF, HGF, bFGF, or EGF driven cords. n = 3 per group. ‡ = p<0.05 vs. BM. † = p<0.05 vs. VEGF. # = p<0.05 vs. bFGF. + = p<0.05 vs. HGF. * = p<0.05 vs. EGF. Scale bars are 250 µm.